Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(D3) | Shigella flexneri |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using PEG 550 MME as precipitant | Shigella flexneri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE | Shigella flexneri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shigella flexneri | - |
- |
- |
Shigella flexneri 2a | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating Sepharose column chromatography, Resource-Q anion exchange chromatography and HiLoad 16/60 Superdex G75 column chromatography | Shigella flexneri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diadenosine tetraphosphate + H2O | - |
Shigella flexneri | 2 adenosine diphosphate | - |
? | |
diadenosine tetraphosphate + H2O | - |
Shigella flexneri 2a | 2 adenosine diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | DynaPro dynamic light scattering | Shigella flexneri |
Synonyms | Comment | Organism |
---|---|---|
diadenosine tetraphosphate hydrolase | - |
Shigella flexneri |